Effect of Chitosan, Coconut Water and Potato Extract on Protocorm Growth and Plantlet Regeneration of Cymbidium aloifolium (L.) Sw.

Effective and rapid in vitro plantlet regeneration from protocorms of Cymbidium aloifolium using chitosan, coconut water potato extract were investigated. Two experiments performed twelve week old size 2-3 mm. In the first experiment, propagated on solid New Dogashima (ND) medium supplemented with various concentrations chitosan (0, 0.05, 0.10 1.00 mg/l). For second cultured same 100, 150 200 ml/l) 5, 10 15 g/l). Survival response percentages, shoot number length, root leaf fresh dry weight collected after weeks culture. The results showed that 0.05 mg/l treatment was most effective for protocorm growth, producing highest number, weight. combination treatments revealed ml/l alone best This gave significantly different other treatments. Findings will provide useful information commercial or conservation orchid propagation programs.